Simona Elena PISCULUNGEANU, Liliana Cristina SOARE, Nicoleta Anca ȘUȚAN, Alina PĂUNESCU, Monica POPESCU, Oana Alexandra LUȚU
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spores, ferns, silver nanoparticles, phytotoxicity.
Currently, silver nanoparticles (AgNPs) have a wide applicability and are being used in more and more fields, including industrial, medical, agricultural, environmental protection, etc. Once in the environment, nanoparticles can have negative effects on living things or neutral or beneficial effects. The aim of this research was to highlight the influence of silver nanoparticles on spore germination and gametophyte differentiation in Asplenium scolopendrium, Athyrium filix-femina, and Dryopteris filix-mas fern species. In the in vitro experiment, AgNPs (Thermo Scientific Chemicals) with a size of 20 nm, 0.02mg/mL, supplied in 2mM sodium citrate, diluted 10 (N1), 100 (N2), and 1000x (N3) were used. The control variants were cultivated on Knop solution (Control 1), as well as on the mixture of Knop solution and 2mM sodium citrate (1:1) (Control 2). The cultures maintained at room temperature (18±2°C) were periodically analyzed microscopically after 3 and 8 weeks, respectively. The results obtained indicate that the AgNPs solution, dilution 10-1, inhibits spore germination in the three tested species. The other two nanoparticle solutions (dilutions 10-2 and 10-3) to which the spores and gametophytes were exposed determined a gametophyte differentiation similar to the control variant.
Cite this paper: Pisculungeanu, S.E., Soare, L.C., Șuțan, N.A., Păunescu, A., Popescu, M., Luțu, O.A., (2025). THE INFLUENCE OF SILVER NANOPARTICLES ON GAMETOPHYTE DIFFERENTIATION IN SOME LEPTOSPORANGIATE FERNS. Current Trends in Natural Sciences, 14(28), 28-35. https://doi.org/10.47068/ctns.2025.v14i28.002
